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血管内皮细胞生长因子在鸡马立克病肝脏肿瘤中的表达

Expression of Vascular Endothelial Cell Growth Factor in the liver tumors of of chickens with Marek's disease
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摘要 为研究血管内皮细胞生长因子(VEGF)在鸡马立克病肝脏肿瘤中的表达,探索其在马立克病(MD)肿瘤形成中的作用与机制。采用实时荧光定量PCR技术、Western blot技术,检测MD病毒组鸡肝脏肿瘤及正常组鸡肝脏中VEGF mRNA和蛋白质的表达。结果表明,MD病毒组鸡肝脏VEGF的mRNA和蛋白表达水平极显著高于正常组(P〈0.01)。表明VEGF参与了MD肿瘤的形成。 The objective of this study was to study the expression of VEGF(Vascular endothelial cell growth factor)in the tu-mors of chickens with Marek's disease,and to explore its role and mechanism in the process of tumor formation in Marek's disease.The mRNA and protein expression levels of active factors in vascular endothelial cells in the liver tumors of chickens with Marek'sdisease and the normal group were examined by Real-time quantitative PCR and Western blot. The Result showed that in the MD liv-er tumors VEGF mRNA and protein were expressed at high levels compared to that of normal group(P0.01),suggesting that VEGF may be involved in the formation of Marek's disease-induced liver tumors.
作者 成慧艳 张斌 詹俊杰 谷尧亮 李宏全 段智变 CHENG Hui-yan , ZHANG-Bin , ZHAN Jun-jie , GU Yao-liang , LI Hong-quan , DUAN Zhi-bian (College of Animal Science and Veterinary Medicine, Shanxi AgricuhuralUniversity, Taigu 030801, China)
出处 《中国兽医杂志》 CAS 北大核心 2014年第9期14-16,共3页 Chinese Journal of Veterinary Medicine
基金 山西省科技攻关项目(20140311022-1) 山西农业大学横向科技项目资助(2013HX28)
关键词 VEGF 鸡马立克病 肿瘤 实时荧光定量PCR WESTERNBLOT VEGF Marek's disease tumor QRT-PCR Western blot
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