期刊文献+

高氟对大鼠甲状腺功能和脑损伤的影响 被引量:7

Influence of high-fluoride on thyroid function and brain damage in rats
收藏 分享 导出
摘要 目的观察高氟对大鼠甲状腺功能和脑损伤的影响。方法将36只成年Wistar大鼠按性别和体质量随机分为3组:对照组、高氟组、高氟+甲状腺片组,每组12只,分别饮用自来水(含氟量〈5mg/L),加入100、100mg/L氟化钠(NaF)的自来水;喂养7个月后高氟+甲状腺片组大鼠另给予0.04%甲状腺片(1.8ml·kg^-1·d^-1)灌胃3周。喂养7个月零3周时,用放射免疫法测定各组大鼠血清TT3、TT4;光镜下观察大鼠甲状腺和脑组织形态结构;免疫组化方法检测海马区域NMDAR2B蛋白表达。结果与对照组[(0.97±0.15)、(84.03±12.45)nmol/L]比较,高氟组大鼠血清TT3、TT4明显降低[(0.24±0.07)、(15.16±2.08)nmol/L,P均〈0.01];高氟+甲状腺片组与对照组比较,未见明显改变[(1.02±0.19)、(85.63±9.55)nmol/L,P均〉0.05],与高氟组相比较,明显增高(P均〈0.01)。光镜下高氟组可见甲状腺部分滤泡上皮增生、排列紊乱、萎缩,滤泡腔内胶质减少,而高氟+甲状腺片组与高氟组比较,甲状腺滤泡上皮增生程度减轻;高氟组海马神经元细胞肿胀、排列紊乱,高氟+甲状腺片组海马神经元细胞较高氟组肿胀减轻、排列有序。高氟组海马CA1、CA3区NMDAR2B阳性细胞灰度值(167.05±7.31)较对照组(92.53±9.67)和高氟+甲状腺片组(101.66±12.21)明显增高(P均〈0.01)。结论高氟可导致成年大鼠甲状腺功能降低、形态改变,并引起大脑病理损害;在高氟损伤基础上给予甲状腺制剂替代后,大鼠甲状腺功能和形态可基本恢复正常,脑组织损伤明显减轻。慢性氟中毒造成的甲状腺功能降低可能是高氟造成脑损伤的另一重要参与因素。 Objective To study the influence of high-fluoride on thyroid function and brain damage. Methods Thirty-six Wistar rats were randomly divided, according to weight and gender into 3 groups (12 rats each), i.e. control group, high fluoride group, and high fluoride plus thyroid tablet treatment group. The rats were fed with normal tap water containing no more than 5 mg/L NaF and the tap water added 100,100 mg/L NaF, respectively. After 7 months of experiment, the rats in high fluoride plus thyroid tablet treatment group were given with 0.04% thyroid tablet(1.8 ml·kg^-1·d^-1) by gastric perfusion for three weeks. The contents of TT3 and TT4 in serum were detected by radio-immunological assay; the histomorphology in thyroids and brains were observed under microscopy; and the protein level of NMDAR2B subunit of glutamate receptor in the hippocampal CA1 and CA3 was measured by immunohistochemistry. Results As compared to the values of TT3 and TT4 in serum of rats in control group[ (0.97 ± 0.15), (84.03 ± 12.45)nmol/L],TT3 and TT4 in high fluoride group were obviously lower [(0.24 ± 0.07), (15.16 ± 2.08)nmo]/L, all P 〈 0.01]; while no changes in TT3 and TT4 were detected in high fluoride plus thyroid tablet treatment group[ (1.02 ± 0.19), (85.63 ± 9.55 )nmol/L, all P 〈 0.05 ] as compared to controls, but higher than those in high fluoride group (all P 〈 0.01 ). The pathological changes including partial hyperplasy, arrangement disorder, atrophy, and decreased colloid of the thyroid follicular epithelial cells in high fluoride group were observed under microscopy. In high fluoride plus thyroid tablet treatment group, the degree of the thyroid cellular hyperplasy was relatively slight as compared to high fluoride group. The swelling and disarrangement of neurons in the hippocampus were observed in high fluoride group, whereas the changes of the neurons were not so obvious in high fluoride plus thyroid tablet treatment group. The grey values of NMDAR2B positive cells in
作者 邱艳红 孔德明 杨勤 赵娜 QIU Yan-hong, KONG De-ming, YANG Qin, ZHAO No. (Department of Endocrinology, the First Affiliated Hospital in Guiyang Traditional Chinese Medical College, Guiyaug 550002, China )
出处 《中国地方病学杂志》 CAS CSCD 北大核心 2010年第2期146-149,共4页 Chinese Jouranl of Endemiology
关键词 甲状腺 脑损伤 海马 Fluoride Thyroid gland Brain injuries Hippocampus
  • 相关文献

参考文献15

二级参考文献107

共引文献108

同被引文献110

引证文献7

二级引证文献18

投稿分析
职称考试

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈